The most definitive, accurate, and reliable method for diagnosing oral mucosal lesions is the scalpel biopsy. The oral brush biopsy coupled with computer-assisted analysis (OralCDx, OralScan Laboratories, Inc., Suffern, NY) has been developed as a technique for evaluating unexplained clinically detectable alterations of the surface epithelium of the oral mucosa whether cancer or pre-cancer is suspected (Sciubba, et al., 2003). The goal of the oral brush biopsy is to provide a highly sensitive and specific technique that is less painful and simpler to perform than a scalpel or punch biopsy.